Sensitive and specific modified Hodge test for KPC and metallo-beta- lactamase detection in Pseudomonas aeruginosa by use of a novel indicator strain, Klebsiella pneumoniae ATCC 700603.
نویسندگان
چکیده
We evaluated the ability of the modified Hodge test to discriminate between KPC- and metallo-beta-lactamase (MBL)-producing Pseudomonas aeruginosa isolates and carbapenemase nonproducers. With Escherichia coli ATCC 25922 as the indicator strain, the MHT resulted in low sensitivity, specificity, and repeatability. Replacing the indicator strain with Klebsiella pneumoniae ATCC 700603 led to an improved performance (100%, 97%, 0%, and 100% sensitivity, specificity, indeterminate results and repeatability, respectively).
منابع مشابه
تشخیص متالوبتالاکتامازها، بتالاکتامازهای با طیف وسیع و پورین‌های غشاء خارجی در ایزوله‌های کلبسیلا پنومونیه جدا شده از بیماران بستری در بیمارستان‌های شهر تهران
Background and Objective: Klebsiella pneumoniae is one of the most important causative agents both in form of hospital acquired and community-acquired infections. This bacterium is one of the most prevalent pathogens that is isolated from hospitals and is associated with high mortality rates. The uprising trend of multi drug resistance among Klebsiella pneumoniae has limited the treatment optio...
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Background & Aims: Imipenem is a member of Carbapenem with stability against most -lactamases .It is of particular use in the treatment of infections associated with drug resistant gram negative bacteria harboring ESBL and AmpC genes. The aim of this study was to determine the imipenem resistance in gram negative bacteria causing nosocomial infections and the presence of metallo--lactamases (MB...
متن کاملفراوانی اینتگرون‌های کلاس I و II در ایزوله‌های بالینی سودوموناس آئروژینوزا مولد متالوبتالاکتاماز
Background and Objective: Pseudomonas aeruginosa is an opportunistic nosocomial pathogen. Evidence suggests that the incidence of enzyme-producing strains of Pseudomonas aeruginosa Metalo Beta Lactamases (MBL) is a major problem in the treatment of infections caused by this organism. The aim of this study was to investigate the frequency of class I and II integrons among metalobetalactamase pro...
متن کاملCombined Use of the Modified Hodge Test and Carbapenemase Inhibition Test for Detection of Carbapenemase-Producing Enterobacteriaceae and Metallo-β-Lactamase-Producing Pseudomonas spp.
BACKGROUND We evaluated the combined use of the modified Hodge test (MHT) and carbapenemase inhibition test (CIT) using phenylboronic acid (PBA) and EDTA to detect carbapenemase-producing Enterobacteriaceae (CPE) and metallo-β-lactamase (MBL)-producing Pseudomonas spp. METHODS A total of 49 isolates of CPE (15 Klebsiella pneumoniae carbapenemase [KPC], 5 Guiana extended-spectrum β-lactamase [...
متن کاملEmergence of blaGES-5 in clinical colistin-only-sensitive (COS) Pseudomonas aeruginosa strain in Brazil.
1. Yigit H, Queenan AM, Anderson GJ et al. Novel carbapenemhydrolyzing b-lactamase, KPC-1, from a carbapenem-resistant strain of Klebsiella pneumoniae. Antimicrob Agents Chemother 2001; 45: 1151–61. 2. Smith Moland E, Hanson ND, Herrera VL et al. Plasmidmediated, carbapenem-hydrolysing b-lactamase, KPC-2, in Klebsiella pneumoniae isolates. J Antimicrob Chemother 2003; 51: 711–4. 3. Yigit H, Que...
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عنوان ژورنال:
- Journal of clinical microbiology
دوره 49 12 شماره
صفحات -
تاریخ انتشار 2011